Assessment of Genetic Polymorphisms in DNA from Formalin Fixed Neurological Tissues
نویسندگان
چکیده
منابع مشابه
Dna Extraction from Formalin Fixed Franciscana Tissues
The present paper reports the extraction of DNA from formalin-fixed Pontoporia blainvillei tissues. Following the Vachot and Monerot (1996) protocol, fragmented DNA (300-700bp) was extracted from more than 95% of liver and muscle samples. DNA yield in liver samples was significantly higher than in muscle samples (4.574 ± 1.169μg DNA/mg versus 0.808 ± 0.297μg DNA/mg). Similar results were obta...
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Therapeutic development is increasingly taking advantage of the expanding field of human genetics. The genetic similarity between humans and non-human primates has increased the importance of the non-human primate as a model to develop new therapeutic modalities. DNA analysis of targeted genetic loci has revealed genetic variation and similarities that underlie observed idiosyncratic and genera...
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BACKGROUND Precision medicine is dependent on identifying actionable mutations in tumors. Accurate detection of mutations is often problematic in formalin-fixed paraffin-embedded (FFPE) tissues. DNA extracted from formalin-fixed tissues is fragmented and also contains DNA lesions that are the sources of sequence artifacts. Sequence artifacts can be difficult to distinguish from true mutations, ...
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ژورنال
عنوان ژورنال: Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques
سال: 1994
ISSN: 0317-1671,2057-0155
DOI: 10.1017/s0317167100041226